![]() No antibody to the external envelope protein was detected for HIV-1 (anti-gp120), whereas antibody to a similar protein (anti-gp 105) was detected in 4.3% of the HIV-2 indeterminate sera. In the Western blot analysis using the WHO criteria, the frequencies of appearance of antibodies to the high molecular weight core proteins of p24 (HIV-1) and p26 (HIV-2) were both quite high (90%). Comparison of HIV-1 and HIV-2 indeterminate Western blot patterns indicated that antibodies to p26 appeared 25% of the time in the negative control sera for HIV-2, whereas no antibodies to HIV-1 products were detected in any of the HIV-1 negative control sera. The GAG gene product p26 reacted with most (91.2%) of the 396 indeterminate sera. Analysis of HIV-2 indeterminate Western blot patterns showed the frequency of appearance of antibodies to the various viral gene products in 396 HIV-2 indeterminate sera. Antibodies for the GAG gene products, i.e., anti-55, anti-p24, and anti-p18 had high frequencies of occurrence with anti-p24 occurring 90.5% of the time. Analysis of HIV-1 indeterminate Western blot patterns showed the frequency of protein bands for 231 HIV-1 indeterminate sera. HIV-2 GAG gene product p26 was shown to be a non-specific indicator of infection. Antibodies to group specific antigen (GAG) gene products were most frequently detected both HIV-1 and HIV-2 indeterminate sera. Sera and plasma after screening by enzyme-linked immunosorbent assay (ELISA) were used. In both cases, proteins of the specific virus type were used to detect anti-HIV proteins in sera by the enzyme linked immunoelectrotransfer blot (Western blot) technique. New LavblotII kits were used for detecting HIV-2 antibodies. For HIV-1 antibodies detection, Novopath Immunoblot assay kits were used. Antibody to gp120, and envelope gene product of HIV-1 never occurred in indeterminate sera whereas antibodies to all the envelope gene products of HIV-2 were detected in indeterminate sera.Ī comparison of HIV-1 and HIV-2 indeterminate Western blot patterns of Ghanaian sera collected between 19 was made and interpreted according to new World Health Organization (WHO) criteria. Gurmukh Singh answered Pathology 51 years experience Negative: If nat was negative at 6 months after exposure you not likely to have hiv. The supplemental HIV 1 and 2 were negative as were the western blot and the. I tested positive in 2005 for anti hiv 1 and 2. ![]() 2 negative oraquick all 1 1/2 year post exposure. HIV test results (PREGNANCY) (INDETERMINATE WESTERN BLOT) Apr 22, 2008. If you think you may have a medical emergency, call your physician or 911 immediately.A comparison of HIV-1 and HIV-2 indeterminate Western blot patterns of Ghanaian sera collected between 19 was made. 1 thank A 22-year-old male asked: Tested false positive elisa hiv test last month. By using this Site you agree to the following Terms and Conditions. During the first four-to-six weeks of Lyme infection, these Lyme disease tests are unreliable because most. The CDC recommends that doctors first order an ELISA to screen for Lyme disease and then confirm Lyme disease with a Western blot. We offer this Site AS IS and without any warranties. The two most-used antibody tests are the enzyme-linked immunosorbent assay (ELISA) and the Western blot. it takes time for immune system to produce antibodies. elisa, ie oraquick, uses only 1 or 2 bands (for hiv-2) of synthetic antigen for antibody detection. Objective To compare human immunodeficiency virus(HIV)positive results. Never disregard the medical advice of your physician or health professional, or delay in seeking such advice, because of something you read on this Site. if 3 bands (parts of the hiv regions) show up in wb, then its positive. of HIV positive results between ELISA screening and western blot testJ. We disclaim all responsibility for the professional qualifications and licensing of, and services provided by, any physician or other health providers posting on or otherwise referred to on this Site and/or any Third Party Site. ![]() MedHelp is not a medical or healthcare provider and your use of this Site does not create a doctor / patient relationship. It is not intended to be and should not be interpreted as medical advice or a diagnosis of any health or fitness problem, condition or disease or a recommendation for a specific test, doctor, care provider, procedure, treatment plan, product, or course of action. The Content on this Site is presented in a summary fashion, and is intended to be used for educational and entertainment purposes only.
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